Regulation of the Wnt signaling pathway during myogenesis by the mammalian SWI/SNF ATPase BRG1

Skeletal muscle differentiation is a tightly regulated process, and the importance of mammalian SWI/SNF (mSWI/SNF) chromatin remodeling family for regulation genes involved in skeletal myogenesis well-established. Our prior work showed that bromodomains mSWI/SNF ATPases BRG1 BRM contribute to by facilitating binding enzymes regulatory regions myogenic other target genes. Here, we report pathway analyses differentially expressed from study identified an additional role via Wnt signaling pathway. The has been previously shown be important development. To investigate pathway, individual dual knockdowns were performed followed RNA-sequencing. results show BRG1, but not BRM, regulator components downstream Reactivation stabilization β-catenin could rescue defect gene expression due knockdown or bromodomain inhibition using specific small molecule inhibitor, PFI-3. These demonstrate required upstream function. Chromatin immunoprecipitation at promoters component β-catenin, which provides further mechanistic insight transcriptional these